Frederik Verweij started his PhD in 2009 in the lab of Dr. Pegtel in Amsterdam where he studied the sorting of a viral oncoprotein into exosomes. Exosomes are a subclass of (small) Extracellular Vesicles that can shuttle biological cargo from one cell to another, able to reprogram the recipient cell. During his PhD, Frederik also pioneered the visualization of exosome secretion from living cells by a TIRF live-imaging approach. In 2014, he started as a Postdoc at the Curie Institute in Paris, France in the lab of Dr. Raposo to complete this work together with Dr. Van Niel, where he used a dynamic Correlative Light-Electron Microscopy approach to demonstrate MVE–PM fusion at supraoptical EM resolution. Supported by an EMBO long-term fellowship, he then studied the physiology of endogenous exosomes in vivo by developing a novel zebrafish embryo model system. Subsequently, he worked as senior researcher in the lab of Dr. Van Niel at the IPNP (Paris) where he used these models to study how inter-organelle contacts tune exosome secretion. Since September 2021, he joined the Center for Living Technologies at the division of Cell Biology, Neurobiology and Biophysics at Utrecht University as PI, where he is leading his group investigating exosome biology by developing smart molecular tools in combination with state-of-the-art (live) imaging techniques.